Welcome to Darkfield Microscope
A Dark Field Microscope is a standard laboratory apparatus to which definite optical strategies are apply to convert the light travels through the specimen being examined. It is a microscope that conducts examination from fresh blood cells to live insects within an extensive scope of specimens.
With the use of a dark field microscope the researchers can study maritime bodies like plankton, algae, bugs, filaments hairs, ferment, protozoa, minerals, crystals, ceramics and thin polymers. Researches on live microbes as well as mounted cells and tissues can also be conducted by using this kind of microscope.
It uses oblique light to increase the visibility of a definite sample. The sample then displays brightly on a black background. This kind of microscope can also examine low antithesis specimens that are not easily viewed within a normal light microscope.
Dark field microscope works with specimens that contain natural pigments. The specimen is requires to be thick enough to take in the incoming light; thus staining is normally used together with this kind of microscope. If there is some instances that the specimen is transparent or in light color like plankton, it will not be noticeable against a sturdy white background. They are unable to assimilate whatever light passes through them, so the specimen show up unseen to the researcher. This is when the notion of dark field illumination take place.
Some of the examples of specimen that is translucent and are often used with dark field illumination are: living bacteria, live blood samples, pollen samples, grass or soil samples, molecules such as caffeine on crystals, aquatic environment samples, organisms with single celled and specimens with light stained transparent.
The dark field microscope has many advantages and disadvantages like other types of microscope. One great advantage of this method is that it can provide quality images with high contrast, extremely easy to use and very successful in showing the features of the specimen being studied.
The major disadvantage of using this microscope is the level of light has a tendency to be low. The excessive bright light can harm the specimens, thus replacing it can be a tough case. There are some particular aspects of the specimen that may be uncertain in a dark field microscope for example, the insect's vein that means the researcher could possibly fail some significant or amusing facts.